Thank-you notes

Hi, everyone!  So, I know the project is a little slow to start up, but hang in there.  We’ll have updates coming your way soon!

In the meantime, I have just finished writing all the thank-you cards and will be shipping out the remaining rewards this week.  If you haven’t gotten your card and/or reward within the next couple of weeks, please drop me an email with your name and address, and I’ll make sure it gets there.

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5 hours left!

Just 5 hours left in our #SciFund Challenge campaign to raise money to test ancient Roman DNA.  In a few short days, the blog and twitter stream will become password-protected.  If you want to keep up on the latest happenings (which will be posted here), you can head over to RocketHub and donate $10 or more.  The funding campaign will close on Thursday at 11:30 eastern time (4:30 AM Friday in GMT), so hurry!

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Romans, DNA, and the Weinersmiths

Kelly Weinersmith, who’s running her own #SciFund Challenge project over on RocketHub on zombie fish, invited me to come on the Weekly Weinersmith podcast that she puts out with her husband, Zach (of Saturday Morning Breakfast Cereal fame).  I enthusiastically accepted and talked to Zach and Kelly for the better part of an hour about the skeletons of ancient Romans.

Click here to go to the podcast!  (Or, you can subscribe via iTunes.)

Feel free to use the comments to start a discussion, give me pointers about interviewing, or ask questions!

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Research Goals

By putting the Roman DNA Project out to the public, I made the choice to focus on the big picture, on what the results will tell me in general about the people of Imperial Rome.  In a grant proposal to an agency like the NSF, NEH, or Wenner-Gren, though, I would have spelled out my hypotheses more fully and in more technical terms.  This post provides a bit more information on the specific goals of the Roman DNA Project, without getting too jargony-grant-speak-y.

Goal 1 – Use mtDNA analysis to more fully understand the demographics of the population of Imperial Rome.  For this pilot phase of the project, we’ll be looking at mitochondrial DNA to learn more about the maternal genetic lines of the Romans – both the locals and the people that previous isotope study suggested were immigrants.  Very broadly, mtDNA (which is passed on, unchanged, from mother to daughter) will give us additional information about the geographic backgrounds of the people buried in the two cemetery populations, contributing further to discussions about demography in Rome.  We expect to find mostly central European haplogroups, but there may be some African, Iberian, and Near Eastern genes.  In combination with the previous Sr/O/Pb (and to some extent C) isotopes, though, we may be able to say with more confidence that a particular person originated in North Africa, Spain, Asia Minor, etc.  Depending on the data produced, we’ll learn more about the origins of the population of Imperial Rome (tracing it back multiple generations) and the origins of specific people at Rome (immigrants or possibly one generation removed).

Goal 2 – Use mtDNA analysis to investigate the genetic diversity of the Republican population of Rome.  In addition to learning more about the genetic background of the Imperial population, we plan to look at a sample of people from the earlier Republican period.  A new theory has been proposed recently about the Roman Republic, with the suggestion that the population was highly mobile rather than sedentary peasants – perhaps even as mobile as people were in the Empire.  Looking at mtDNA from the Republican samples (combined with previous Sr/O isotopes) will tell us a lot about the geographic origins of the population, namely whether there was significant gene influx prior to the Empire.  Granted, the Republican sample is a small one, but the data that result should be of great interest to classical historians and demographers and will pave the way for future studies of Republican-era skeletons.

Goal 3 – Use mtDNA analysis to learn more about female mobility in the Empire.  We’re also choosing mtDNA for this pilot phase specifically because it’s inherited from one’s mother.  The mobility of females is a popular question in classics – many people think that women weren’t often taken as slaves and that they didn’t have the freedom to move like men did.  My recent EAA paper compared Sr/O data from M1s and M3s of female immigrants to Rome; I found that women did move, and that some moved multiple times during their lives.  Another interesting question that this DNA project will start to answer is about genetic diversity among males and females.  The Roman family was often neolocal in residence (the nuclear family lived separately) but patrilocal in burial (people were buried with their father’s/father-in-law’s family).  In a Roman burial context, we might expect more genetic diversity in females who were marrying into a family.  But more men moved around the Empire because of the military and slavery, so a cemetery population may have more diversity in males, especially if the cemetery was specifically for slaves.  Eventually, it would be great to do some Y-DNA testing, to look into the genetic diversity of males as well.  But the mtDNA will give us a good head-start on answering questions of sex-specific mobility.

Future Goal – Use DNA, isotope, and palaeopathological analysis to answer questions about disease ecology.  Malaria has long been a topic of interest to classicists; some DNA work has been done in the past (see, for example, Sallares’ 2002 book Malaria and Rome) and shows that it’s possible to identify P. falciparum, the organism that causes malaria, within a skeleton.  DNA analysis, though, could also let me look for genetic anemias like thalassemia, sickle-cell, and G6PD, which confer some protection from malaria and were likely common in the Mediterranean.  Interestingly, I recently charted the oxgyen isotope ratios for people with and without porotic hyperostosis, a bone pathology resulting from anemia, and there is a statistical correlation between high oxygen ratios and presence of porotic hyperostosis.  Higher oxygen ratios mean warmer climates, and may also mean geographical areas with higher (or different) parasite loads.  There’s definitely promise in looking at the disease ecology of Rome/Italy at a molecular level, so I’m hoping that we successfully extract DNA in this pilot phase.

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We’re funded!

Thanks to an amazing piece at the CNN blog Light Years by Ed Yong, the outpouring of support for the Roman DNA Project today has been astounding!  In financial news, we have actually exceeded our $6,000 goal, after just 10 days.  That goal was to fund analysis of at least 20 individuals (the immigrants to Rome that I found through Sr/O isotope analysis).  Of course, we are accepting donations through mid-December, so additional funding will be put to good use – studying more ancient Romans!

And I’ve received a dozen or more emails today from people as excited as I am about this project, offering their encouragement, lab services, expertise, and knowledge about the ancient world.  I will respond to all of them, I promise, but it might take a few days!

Again, thank you – all of you reading this – for making this project a reality!

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Were these people Romans?

In short: maybe.  “Roman” was a legal status, the status of a citizen, and the rules for citizenship varied depending on time and place in the Empire.  During the time period that saw the burials at Casal Bertone and Castellaccio Europarco, perhaps up to 35% of the population of Rome were slaves, and many of those probably arrived at Rome from somewhere else.  Slaves were definitely not Roman, because they were not citizens, even after emancipation.

The people whose DNA we will study are an unknown population: without inscriptions, without historical records, without grave goods, all that can be said is they were of the lower-class, which composed just under 99% of the population of Rome.  These were average people – men, women, children… free, enslaved, freed… workers, parents, subjects of Imperial power.

The title of this website – the Roman DNA Project – is then a bit of a misnomer.  We’re not going to know if we’ve found “Roman” DNA, but it’s also impossible to say what “Roman” DNA would be since that changed quite often over the duration of the Republic and Empire.  What we will get is DNA from people buried at Rome, which is pretty cool in itself.

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We’re big in the Netherlands!

Lucas Brouwers wrote about the #SciFund Challenge and highlighted my project in particular in his piece at the Dutch newspaper NRC Handelsblad!  Thanks to this coverage, the project has already started receiving donations from interested folks in the Netherlands.

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